【ACE C18色谱柱】LC-MS/MS法测定血浆中的原霉素组分分析

【ACE C18色谱柱】LC-MS/MS法测定血浆中的原霉素组分分析

Pristinamycin components in plasma by LC-MS/MS

ACE 3 C18 3μm, 30 x 3.0mm
Gradient analysis
A = 1mM NH4CO2H + 0.1%
HCO2H in 65:35 H2O:CH3CN
B = CH3CN
T (mins) %B T (mins) %B
0 0 1.61 100
0.3 0 2.6 100
0.31 10 2.61 0
1.6 10 4 0
Flow rate: 1ml/min
Column temperature: 25°C
Injection volume: 10μl
MDS Sciex API 4000
TurboIonSpray positive mode

Transitions monitored:
Pristinamycin lA 867.5 → 134.2
Pristinamycin llA 526.3 → 355.1
I.S. (Virginiamycin) 824.6 → 134.0
Pristinamycin antibiotic is a mixture of 2 components –pristinamycin lA and llA
Virginiamycin used as internal standard